2×Hotmaster PcR mastermix (with loading dye), Innovative and Unique Hot-Start / cold-Stop taq DNA Polymerase, Quantity 1ml, KT208-01

Description：

HotMaster Taq DNA Polymerase is a superior alternative for performing PCR
experiments generally known as “hot start” PCR. HotMaster blocks the substrate
binding site of DNA polymerases in a temperature-dependent manner. Inactive
polymerase-inhibitor complexes are formed at temperatures < 40°C, where the
affinity of HotMaster for Taq polymerase is higher than the binding affinity of
the template DNA . Between 40°C and 55°C the HotMaster competes with the
template DNA for binding to the Taq polymerase, thereby shifting the binding
equilibrium towards complex formation with only target-specific primed template
DNA . At temperatures above 55°C the HotMaster inhibitor is displaced from
complexes with the Taq polymerase by target-specific primed template DNA. A
unique performance feature of the HotMaster inhibitor is that it can go through
multiple temperature cycles of binding-equilibrium competition-dissociation
during PCR without irreversible heat inactivation. Where other Taq polymerase
formulations for “hot start” PCR block the activity of Taq polymerase only prior
to the first high temperature step, the TIANGEN HotMaster provides sustained
temperature control throughout PCR.

Definition of Activity Unit：
One unit is defined as the amount of the enzyme required to catalyze the
incorporation of 10 nmoles of dNT P’s into an acid-insoluble form in 30 minutes at
74°C using hering sperm DNA as substrate.

Quality Control：
The absence of endodeoxyribonucleases, exodeoxyribonucleases and ribonucleases.

Size/Quantity :

1ml